| Registro completo |
| Provedor de dados: |
Neotropical Ichthyology
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| País: |
Brazil
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| Título: |
Fresh, equilibrated and post-thaw sperm quality of Brycon orbignyanus (Valenciennes, 1850) and Prochilodus lineatus (Valenciennes, 1837) treated with either salmon GnRHa and domperidone or pituitary extract
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| Autores: |
Viveiros,Ana T.M.
Gonçalves,Antonio C.S.
Nascimento,Ariane F.
Leal,Marcelo C.
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| Data: |
2015-03-01
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| Ano: |
2015
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| Palavras-chave: |
CASA
Characiformes
Cryopreservation
Ovaprim™
Sperm motility
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| Resumo: |
The effects of reduced doses of Ovaprim™ (GnRHa + domperidone) on sperm release of Brycon orbignyanus and Prochilodus lineatus were evaluated. Furthermore, sperm quality was compared among fresh, equilibrated and post-thaw samples. Males received a single and reduced dose of Ovaprim™ (0.125 or 0.25 ml/kg); control males received pituitary extract (cPE; 3 mg/kg). Fresh sperm was evaluated for volume, concentration, seminal plasma osmolality and seminal plasma pH. Then sperm was diluted in a freezing medium, equilibrated for 15-20 min and frozen in nitrogen vapor vessel (dry-shipper). Sperm motility was analyzed during 60 s post-activation in fresh, equilibrated and post-thaw samples. Sperm quality of males treated with Ovaprim™ (both doses) were not different from that of cPE-treated males, thus these data were pooled. In B. orbignyanus, motility was higher in fresh (99%) than in equilibrated sperm (81%); post-thaw motility dropped to 42%. In P. lineatus, motility was similar in fresh (99%) and equilibrated sperm (92%); post-thaw motility was 73%. Motility decreased as a function of time post-activation, and this decrease was significant after 60 s in fresh and equilibrated sperm, and as soon as 30 s in post-thaw sperm, in both species. Ovaprim™ at 1/4 of the recommended dose can successfully replace cPE.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252015000100157
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| Editor: |
Sociedade Brasileira de Ictiologia
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| Relação: |
10.1590/1982-0224-20140066
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| Formato: |
text/html
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| Fonte: |
Neotropical Ichthyology v.13 n.1 2015
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| Direitos: |
info:eu-repo/semantics/openAccess
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